Source: Purified from an E.coli strain carrying Taq-DNA-Polymerase overproducing plasmid. The original enzyme has been isolated from Thermus aquaticus.
Associated activities: Taq-DNA-Polymerase is a highly processive 5’ --> 3’-DNA-polymerase with 5’ --> 3’-exonuclease activity. 3’ --> 5’-exonuclease activity lacks completely. Additionally, the enzyme adds nucleotides (mostly adenosines) to the 3´-ends of the DNA, so that TA-cloning is possible without further modifications.
Application & Quality control:
Primer extension reaction: the enzyme is free of nicking and primer extension activities as well as of exonucleases and unspecific endonucleases. SDS/PAGE: 95 kD-band, purity >98%. Activity and stability tested via PCR. The error rate per nucleotide per cycle is ~8,3x10 -5; the accuracy ~ 1,2x10 4. Estimated half life at 95ºC: 90 min.
Amount: 500 Units, Concentration: 5 µl/U, Volume: 100 µl
incl. buffer set