Taq-DNA-Polymerase

Source: Purified from an E.coli strain carrying Taq-DNA-Polymerase overproducing plasmid. The original enzyme has been isolated from Thermus aquaticus.

 

Associated activities: Taq-DNA-Polymerase is a highly processive 5’ --> 3’-DNA-polymerase with 5’ --> 3’-exonuclease activity. 3’ --> 5’-exonuclease activity lacks completely. Additionally, the enzyme adds nucleotides (mostly adenosines) to the 3´-ends of the DNA, so that TA-cloning is possible without further modifications.

 

Application & Quality control:

Primer extension reaction: the enzyme is free of nicking and primer extension activities as well as of exonucleases and unspecific endonucleases. SDS/PAGE: 95 kD-band, purity >98%. Activity and stability tested via PCR. The error rate per nucleotide per cycle is ~8,3x10 -5; the accuracy ~ 1,2x10 4. Estimated half life at 95ºC: 90 min.


Taq-DNA-Polymerase

Amount: 500 Units, Concentration: 5 µl/U, Volume: 100 µl

incl. buffer set

95,00 €

  • verfügbar
  • 1 - 3 Tage Lieferzeit1

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Data Sheet
DB Taq-DNA-Polymerase.pdf
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