PCR-Mastermix


One.Step RT-PCR Probe Kit

One.Step RT-PCR Probe Kit

 

The enzyme mix is based on a new formulated reverse transcriptase, a Hot-Start Polymerase and RNase Inhibitor. The set is providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The reaction buffer includes extrapure dNTPs and reaction enhancers. The basis is a Real-time PCR with dual labeled probes and multiplexing capacity.

 

Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler

 

Components of Maximo.OneStep RT-qPCR (for probes): Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer, extra pure dNTPs RNase-free water

 

Storage: -20°C, avoid frequent thawing and freezing

 

Transport: the product will be shipped with "blue ice

 

For optimal results it is recommended to make an individually optimization for each RNA / primer pair.

Components: Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer, extra pure dNTPsRNase-free water

 

one.step PCR-Mastermix

415,00 €

  • verfügbar
  • 1 - 3 Tage Lieferzeit1

PCR-Mastermix

PCR-Mastermix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water).

 

Reagent Composition PCR-Mastermix:

- Taq-DNA-Polymerase

- 5 x Reaction Buffer B (400 mM Tris-HCl pH 9,4 – 9,5 at 25°C, 100 mM (NH4)2SO4, 0,1%

  w/v Tween-20)

- 12,5 mM MgCl2, (= 2,5 mM in 1x solution)

- 1 mM dNTPs of each : 1 x PCR solution – dATP, dGTP, dCCT und dTTP (= 200 µM/dNTP in

   1x solution)

 

Source: Purified from an E.coli strain carrying Taq-DNA-Polymerase overproducing plasmid. The original enzyme has been isolated from Thermus aquaticus.

 

Associated activities: Taq-DNA-Polymerase is a highly processive 5’ --> 3’-DNA-polymerase with 5’ --> 3’-exonuclease activity. 3’ --> 5’-exonuclease activity lacks completely. Additionally, the enzyme adds nucleotides (mostly adenosines) to the 3´-ends of the DNA, so that TA-cloning is possible without further modifications.

 

Application & Quality control:

Primer extension reaction: the enzyme is free of nicking and primer extension activities as well as of exonucleases and unspecific endonucleases. SDS/PAGE: 95 kD-band, purity >98%. Activity and stability tested via PCR. The error rate per nucleotide per cycle is ~8,3x10 -5; the accuracy ~ 1,2x10 4. Estimated half life at 95ºC: 90 min.

 


PCR-Mastermix

Volume: 1000 µl or 5000 µl

49,00 €

  • verfügbar
  • 1 - 3 Tage Lieferzeit1

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Data Sheet
DB PCR-Mastermix.pdf
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