HotStart- PCR-Mastermix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water).
Reagent Composition: Hot-Start PCR-Mastermix:
- Hot-Start Taq-DNA-Polymerase
- 5x reagent buffer
- 7,5 mM MgCl2, (= 1,5 mM in 1x solution)
- 2 mM of each dATP, dGTP, dCCT and dTTP (= 200 µM à dNTP in 1x solution)
Source: Purified from an E.coli strain carrying Taq-DNA-Polymerase overproducing plasmid. The original enzyme has been isolated from Thermus aquaticus.
Associated activities: Taq-DNA-Polymerase is a highly processive 5’ --> 3’-DNA-polymerase with 5’ --> 3’-exonuclease activity. 3’ --> 5’-exonuclease activity lacks completely. Additionally, the enzyme adds nucleotides (mostly adenosines) to the 3´-ends of the DNA, so that TA-cloning is possible without further modifications.
Volume: 1000 µl or 5000 µl