HotStart- PCR-Mastermix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water).


Reagent Composition: Hot-Start PCR-Mastermix:


- Hot-Start Taq-DNA-Polymerase

- 5x reagent buffer

- 7,5 mM MgCl2, (= 1,5 mM in 1x solution)

- 2 mM of each dATP, dGTP, dCCT and dTTP (= 200 µM à dNTP in 1x solution)



Source: Purified from an E.coli strain carrying Taq-DNA-Polymerase overproducing plasmid. The original enzyme has been isolated from Thermus aquaticus.


Associated activities: Taq-DNA-Polymerase is a highly processive 5’ --> 3’-DNA-polymerase with 5’ --> 3’-exonuclease activity. 3’ --> 5’-exonuclease activity lacks completely. Additionally, the enzyme adds nucleotides (mostly adenosines) to the 3´-ends of the DNA, so that TA-cloning is possible without further modifications.


Volume: 1000 µl or 5000 µl

172,55 €

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Data Sheet
DB HotStart Mastermix.pdf
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